<i>ZNF367</i> Inhibits Cancer Progression and Is Targeted by miR-195

<div><p>Background</p><p>Several members of the zinc finger protein family have been recently shown to have a role in cancer initiation and progression. Zinc finger protein 367 (<i>ZNF367</i>) is a member of the zinc finger protein family and is expressed in embryonic or fetal erythroid tissue but is absent in normal adult tissue.</p><p>Methodology/Principal Findings</p><p>We show that <i>ZNF367</i> is overexpressed in adrenocortical carcinoma, malignant pheochromocytoma/paraganglioma and thyroid cancer as compared to normal tissue and benign tumors. Using both functional knockdown and ectopic overexpression in multiple cell lines, we show that <i>ZNF367</i> inhibits cellular proliferation, invasion, migration, and adhesion to extracellular proteins <i>in vitro</i> and <i>in vivo</i>. Integrated gene and microRNA expression analyses showed an inverse correlation between <i>ZNF367</i> and miR-195 expression. Luciferase assays demonstrated that miR-195 directly regulates <i>ZNF367</i> expression and that miR-195 regulates cellular invasion. Moreover, integrin alpha 3 (<i>ITGA3</i>) expression was regulated by <i>ZNF367</i>.</p><p>Conclusions/Significance</p><p>Our findings taken together suggest that <i>ZNF367</i> regulates cancer progression.</p>