LRP deficiency in MΦ's results in normal IFN-γ response but decreased IL-4.

<p><b>(A)</b> Mice were challenged with 4 µg αGC/mouse and serum assayed for IFN-γ and IL-4 by ELISA. Data points show standard error and mean. WT (n = 4 and 6 mice for 12 and 24 hour, respectively) and LRP-cKO (n = 5 and 4 for 12 and 24 hours, respectively), each point represents an individual mouse from a representative experiment of three separate experiments <b>(B)</b> WT (n = 7) and LRP-cKO mice (n = 8) challenged with 4 µg αGC/mouse or vehicle control. Intracellular cytokine stain was performed for IL-4 and antibodies for TCRβ, B220, and CD1d-αGC-tetramer to select iNKT cells. iNKT cells were defined as TCRβ^intermediate, B220^-, CD1d-αGC-tetramer⁺. Each point represents an individual mouse from a representative experiment of three separate experiments. <b>(C)</b> Chimeric mice were generated by transplanting Thy1.1 and LRP-cKO (Thy1.2) at a 1∶1 ratio into LysMCre^-/- LRP^flox/flox (Thy1.2) recipient mice. Isolation of splenic iNKT cells and intracellular cytokine stain of IL-4 was performed 4 weeks after transplantation. Dot plots on the left show percentage of Thy1.1⁺ or Thy1.2⁺ iNKT cells expressing IL-4. Graph on the right shows quantification of IL-4⁺ iNKT cells. Data presented from this experiment is the compilation of two separate experiments where each data point represents a single mouse. Bars show standard error and mean. *** denotes <i>p</i><0.0001. <i>P</i>-value determined by Student's <i>t</i>-test.