<p>(Left, A–E) Z-projections of confocal stacks encompassing the developing SC in <i>Prox1-GFP</i> eyes. (A) At P4.5 VECAD labeling shows multicellular sprouts (*) at the sides of the GFP + tubular rSC. These sprouts have no detectable <i>Prox1</i> expression. (B) By P5, remodeling has formed a central core of PROX1<sup>+</sup> flattened cells without any discernible internal space or RBCs. Further development continues by sprouting (*, <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001912#pbio.1001912.s013" target="_blank">Figure S13</a>) from the central core. Dotted line delineates developing SC from autofluorescence arising from nearby tissue. (C) At P10, developing SC has expanded considerably in size, and m...
<p><b>A</b>) Still images of DIC live-cell microscopy (movie S14). Arrowheads indicate filopodia-lik...
(A) Schematic of vertebrate retinal development. After the optic vesicle forms the optic cup, cells ...
<p>(A) Fucci (fluorescent ubiquitination-based cell cycle indicator) labels nuclei of G1 phase cells...
<p>Z-projection of confocal stacks encompassing the rSC from a <i>Prox1-GFP</i> eye at P4.5. VECAD i...
<p>The images show VECAD (yellow) immunolabeled cells in the IZ in the plane of the future SC at the...
<p>(A) Cartoon representing an early stage in the development of SC. At P1 and P2, endothelial cells...
Transverse sections of unablated larvae stained for the RPE marker ZPR2 (A), R/G cone photoreceptor ...
Growing retinal stem cell (RSC) clonal spheres in traditional static culture is associated with low ...
<p><b>(</b>A–D) A model representing four phases of SIV plexus development in the zebrafish embryo b...
<p>(A) Panoramic YZ view of a limbal segment of a <i>Kdr-GFP</i> eye at P3. GFP<sup>+</sup> cell clu...
<p>(A–C) Z-projections of the presumptive SC plane at the indicated ages. The top panels at each age...
A quiescent population of retinal stem cells (RSCs) in the adult mammalian eye retains the ability t...
<p>(A) Marker expression as determined by GFP expression (green, transgenic mice; see Methods) or im...
<p>(A–B) Tip cells from the LVP penetrating into the deeper limbal tissue. (A) Depth coded maximum i...
<p>Z-projections of high magnification confocal micrographs illustrating patterns of HxBP labeling i...
<p><b>A</b>) Still images of DIC live-cell microscopy (movie S14). Arrowheads indicate filopodia-lik...
(A) Schematic of vertebrate retinal development. After the optic vesicle forms the optic cup, cells ...
<p>(A) Fucci (fluorescent ubiquitination-based cell cycle indicator) labels nuclei of G1 phase cells...
<p>Z-projection of confocal stacks encompassing the rSC from a <i>Prox1-GFP</i> eye at P4.5. VECAD i...
<p>The images show VECAD (yellow) immunolabeled cells in the IZ in the plane of the future SC at the...
<p>(A) Cartoon representing an early stage in the development of SC. At P1 and P2, endothelial cells...
Transverse sections of unablated larvae stained for the RPE marker ZPR2 (A), R/G cone photoreceptor ...
Growing retinal stem cell (RSC) clonal spheres in traditional static culture is associated with low ...
<p><b>(</b>A–D) A model representing four phases of SIV plexus development in the zebrafish embryo b...
<p>(A) Panoramic YZ view of a limbal segment of a <i>Kdr-GFP</i> eye at P3. GFP<sup>+</sup> cell clu...
<p>(A–C) Z-projections of the presumptive SC plane at the indicated ages. The top panels at each age...
A quiescent population of retinal stem cells (RSCs) in the adult mammalian eye retains the ability t...
<p>(A) Marker expression as determined by GFP expression (green, transgenic mice; see Methods) or im...
<p>(A–B) Tip cells from the LVP penetrating into the deeper limbal tissue. (A) Depth coded maximum i...
<p>Z-projections of high magnification confocal micrographs illustrating patterns of HxBP labeling i...
<p><b>A</b>) Still images of DIC live-cell microscopy (movie S14). Arrowheads indicate filopodia-lik...
(A) Schematic of vertebrate retinal development. After the optic vesicle forms the optic cup, cells ...
<p>(A) Fucci (fluorescent ubiquitination-based cell cycle indicator) labels nuclei of G1 phase cells...