Flagellar localization of <i>Tb</i>PGKL.

<p>(<b>A</b>) Indirect immunofluorescence using monoclonal antibody BB2 reveals axonemal localization of Ty::<i>Tb</i>PGKL in detergent-extracted procyclic <i>T. brucei</i> cytoskeletons. Cytoskeletons were stained with 4′,6-diamidino-2-phenylindole (DAPI) to detect mitochondrial (kinetoplast, K) and nuclear (N) DNA. The inset shows how the indirect immunofluorescence signal extends close to the kinetoplast, consistent with axoneme association. Scale bar denotes 5 µm. (<b>B</b>) Immunoblot analysis of detergent- and NaCl-extracted flagella isolated from procyclic cells expressing Ty::<i>Tb</i>PGK-like protein using BB2 detects a single band of the expected molecular mass.