Proinflammatory properties of colonic crypt supernatants of CPE-deficient mice.

<p>(A) Experimental set-up for the acquirement and utilization of forskolin-stimulated supernatants of isolated colonic crypts. (B–C) Determination of KC (B) and IL-6 (C) transcript levels produced in MODE-K cells after incubation with LPS (50 ng/ml) and forskolin-stimulated supernatants of <i>cpe</i>^+/+ and <i>cpe</i>^−/− colonic crypts via RT-PCR. (D). KC transcript levels produced in MODE-K cells after incubation with forskolin-stimulated supernatants of of <i>cpe</i>^+/+ and <i>cpe</i>^−/− mice and LPS together with recombinant NPY +/− PYY (1 µM/ml). KC expression levels are expressed in percent of the Mean of <i>cpe^+/+</i>. (E–F) BMDM migration via Boyden chamber assay. Representative pictures of migrated BMDM (E) and quantification (F) of BMDM migration towards supernatants of forskolin-stimulated colonic crypts of <i>cpe</i>^+/+ and <i>cpe</i>^−/− mice. *p<0.05, **p<0.01, ***p<0.001 by t-test.