HIV-1 infection induces a broader range of HIV-1 Env-specific IgG than IgA antibodies in vaginal secretions.

<p>Vaginal secretions were collected from 5 HIV-1 infected women and analyzed for IgG (A–B) and IgA (C–D) binding to the indicated HIV-1 Env antigens. Antigens not specified as Clade C or consensus (Con6 and ConS) are derived from Clade B viruses. (A) Average HIV-1 antigen-specific IgG activity from the combination of the three sampling devices and two time points, calculated as antigen-specific MFI/ng ml⁻¹ total IgG. Black symbols represent negative responses and colored symbols indicate positive responses, with the colors corresponding to specific individuals. Samples that did not meet the positivity criteria are displayed at zero for specific activity. (B) Percentage of women with positive IgG binding. (C) Average HIV-1 antigen-specific IgA activity, calculated as antigen-specific MFI/ng ml⁻¹ total IgA. (D) Percentage of women with positive IgA binding.