Effects of temperature and pH on the activity of HSP hydroxylase from A. <i>tumefaciens</i> S33.

<p>(a) The reactions were carried out in 50 mM Tris-HCl (pH 8.0) at the temperature indicated. (b) The reactions were performed at 30°C in different buffers: citric acid-sodium hydrogen phosphate (pH 5.0 and pH 6.0), sodium phosphate (pH 6.0, pH 7.0 and pH 8.0), Tris-HCl (pH 8.0, pH 9.0 and pH 10.0) and sodium bicarbonate-sodium hydroxide (pH 10.0 and pH 11.0). The values are means of three replicates, and the error bars indicate the standard deviations.