MED23 null single positive thymocytes express decreased KLF2.

<p><b>A–C</b>, <i>Klf2</i> (<b>A</b>), <i>Cd52</i> (<b>B</b>) and <i>S1pr1</i> (<b>C</b>) mRNA expression in CD4⁺ single positive thymocytes; YFP⁺<i>Med23^+/+;Lck-Cre;YFP</i> or <i>Med23^+/+;Lck-Cre</i> (control) and YFP⁺<i>Med23^flox/flox;Lck-Cre;YFP</i> or <i>Med23^flox/flox;Lck-Cre</i> where deletion was greater than 90% (MED23 null) thymocytes. Two-tailed t test; N = 4–8; Mean +/− SEM. <b>D</b>, Western blot of MED23, KLF2 and ACTB (loading control) in whole cell extracts from YFP⁺ CD4 SP SP thymocytes from <i>Med23^+/+;Lck-Cre;YFP</i> (control) and <i>Med23^flox/flox;Lck-Cre;YFP</i> (mutant) mice.