Increased HIV-1 transcription in the brains of METH-user HIV seropositive subjects.

<p>The content of HIV DNA and cellular HIV RNA was profiled in the frontal cortex area of HIV seropositive individuals with and without history of METH use, by real-time PCR and normalized to Actin or GAPDH levels respectively. <b><i>A.</i></b> and <b><i>B.</i></b> HIV-1 DNA and cellular HIV RNA did not show significant changes among groups. <b><i>C.</i></b> HIV-1 RNA/DNA ratio (as a marker of average HIV transcription) was significantly higher in METH user group, suggesting increases on viral transcription in the brain. *p<0.05 by Mann Whitney pairs test.