Long-chain fatty acid oxidation is required for <i>P. aeruginosa</i> virulence and fitness in wounds.

<p>(A) Fold change gene expression (above) or mutant abundance (below) in murine wound infections as compared to MOPS-succinate (Succ.) is shown for the long-chain fatty acid oxidation genes <i>faoAB</i> (*, P<0.05, negative binomial test; †, P<0.01, negative binomial test) (B) Kaplan-Meier survival curves of burned mice infected with wild-type PAO1, an <i>faoA</i> transposon mutant derivative (<i>faoA</i>::Tn), or an unmarked, in-frame <i>faoA</i> deletion mutant (Δ<i>faoA</i>). The experiment was performed twice (PAO1, <i>faoA</i>::Tn) or once (Δ<i>faoA</i>), with three to five mice per group, and the percent survival of all mice is shown (*, P<0.005, log-rank (Mantel-Cox) test; n = 8 (PAO1), n = 10 (<i>faoA</i>::Tn), n = 5 (Δ<i>faoA</i>)). (C) Growth of wild-type PAO1, <i>faoA</i>::Tn, or Δ<i>faoA</i> in murine chronic wounds four days post infection. Each symbol represents a value obtained from infection of an individual mouse. The central bar indicates the mean, and error bars indicate standard error of the mean (*, P = 0.023, unpaired T-test; †, P = 0.091, unpaired T-test).