Functional effects of 10E8 on HIV-1 clade C variants including those with a naturally occurring L673 residue.

<p>(<b>A</b>) Partial neutralization by 10E8 of clade C isolates TM20.13 and M20490 BMR 211 using the TZM-bl assay. (<b>B</b>) Effect of the presence of 10E8 at a saturating concentration (10 µg/ml) on thermostability (T₉₀) of clade C isolates or cognate F673 mutants. Hash tag (#) indicates insufficient infectivity due to lability or neutralization. (<b>C</b>) Percentage of maximum neutralization by 10E8 of various clade C isolates or cognate F673 mutants. (<b>D</b>) Effect of the presence of 10E8 on neutralization sensitivity of clade C isolates or cognate F673 mutants to various ligands. Fold change in IC₅₀ was calculated using the equation: (IC₅₀ without 10E8/IC₅₀ with 10E8). Purple highlight shows when neutralization potency is decreased by greater than three-fold and gold highlight shows when neutralization potency is increased greater than three-fold. <i>nd</i>, not determined.