Dual pulse protocol.

<p><b>A</b>: Simplified schematic representations of the protocols corresponding to a conventional single pulse labelling with one nucleoside analog (e.g., BrdU) and a dual pulse labelling experiment with two different nucleoside analogs (e.g., BrdU together IdU or EdU). <b>B</b>: Artificial staining of single-pulse labelling data (for original data see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003616#pcbi-1003616-g002" target="_blank">Fig. 2</a>), showing eight of the nine subpopulations that could potentially be identified with double-pulse labelling. Notice that the four population and that can be followed by the conventional protocol, have each been subdivided according to the cell cycle phases. The naming convention for the populations is as follows: the superscript ( = ‘labelled undivided’, = ‘labelled divided’, = ‘unlabelled’) indicates whether the population is labelled and whether it has divided since the time of the first pulse; the first and the second subscript () stand for the phase in which the population was at the time of the first and the second pulse respectively. Double subscripts are used only when necessary.