Comparison of the substrate-bound HSP70 SBD to other DnaK and HSC70 SBD structures.

<p>A. Comparison of the NRLLLTG-bound HSP70 SBD to the NRLLLTG-bound DnaK SBD (PDB code 1DKZ). HSP70 and DnaK are shown in magenta and blue cartoons, respectively. The corresponding peptide substrates are shown as stick models. B. A close-up view of the hydrophobic interactions within the helical bundle region of the α subdomain. Hydrophobic residues are shown as stick models. C. Comparison of the SBDα subdomain of the human HSP70-NRLLLTG structure with the isolated three-helix bundle region of the same protein in X-ray structure (PDB code 3LOF, blue) and NMR solution structure (PDB code 2LMG, green). D, Structural alignment of the SBDα subdomain of the HSP70-NRLLLTG structure with the three-helix bundle region of <i>C. elegans</i> Hsp70 (PDB code 2P32, cyan) and DnaK (PDB code 1DKZ, blue). E. Superposition of the lid subdomains of human HSP70 and rat HSC70. HSP70 (MolA) and HSC70 are colored in magenta and cyan, respectively. F. A close-up view of the interaction between the SBDα and SBDβ subdomains of HSP70. Interacting residues are highlighted as sticks, and hydrogen bonds are indicated with dotted lines.