Representative imaging of four distributions and organization of microtubules and spindles in mouse IVM oocytes from maturation cultures of different duration were revealed by confocal microscopy.

<p>(A1–3) Normal spindle of rabbit oocyte with chromosomes arrayed at the metaphase plate. (B1–3 and C1–3) Abnormal spindle with disrupted microtubule bundles. (D1–3) Abnormal spindle with dispersed chromosomes. Column 1 shows the microtubule morphology by α-tubulin staining, Column 2 shows the nuclear morphology by Propidium iodide and Column 3 shows the merged images of spindle. Bar = 7.5 µm