Chronic Δ⁹-THC decreases serum IFN-γ and IL-12p40 levels in the secondary systemic <i>C. albicans</i> infection.

<p>Female c57BL/6 mice (n = 5–7/group) were given vehicle or Δ⁹-THC at 8 mg/kg or 16 mg/kg on days 1–4, 8–11 and 15–18. Mice were injected with <i>C. albicans</i> (7.5×10⁴ yeast cells/ms, i.v.) on day 2 and challenged again with <i>C. albicans</i> (5×10⁵ yeast cells/ms, i.v.) on day 19. Six, twelve or twenty four hours after receiving the second <i>C.albicans</i> challenge, blood was collected for IFN-γ (A) and IL-12 (B) analysis via ELISA. For (A) ^ap≤0.013 compared to the corresponding time point for vehicle treated mice (VEH); ^bp≤0.003 compared to the corresponding time point for VEH; ^cp≤0.0002 compared to the corresponding time point for VEH; Statistical analysis: one way ANOVA followed by Tukey’s multiple comparison test within time points; <b>⁺</b>p≤0.04 compared to VEH mice at 6 h and at 12 h; Statistical analysis: one way ANOVA followed by Dunnett’s. For (B) ^ap≤0.03 compared to the corresponding time point for VEH; ^bp≤0.0001 compared to the corresponding time point or VEH; Statistical analysis: one way ANOVA followed by Tukey’s multiple comparison test within time points. Each symbol in the Figures represents one mouse. This data is representative of two experiments.