Oligomerization of CD27L in solution for wild-type and the M186P and C238R mutants.

<p>A. Size exclusion chromatography elution profile of the UV trace at 280-type CD27L. There are three peaks that correspond to the dimer (68±4 kDa), a mixture of monomer and cleaved C-terminal domain (43±2 kDa), and the monomer (33±7 kDa). B. Elution profile of the CD27L C238R mutant, showing a single peak with a dimeric species (62 kDa). C. Superposition of the elution profiles of the wild-type and the C238R and M186P mutants, showing the relative peak heights at equivalent amounts of sample loaded, with increased aggregation for the M186P mutant. D. Superposition of the elution profiles of wild-type CTP1L (in blue) and the CTP1L mutant D215A (in red).