The transgenic cassette and identification of transgenic zebrafish.

<p>A. Design of the transgenic cassette. <i>m3ck</i> was driven by the skeletal muscle-specific promoter <i>smyd1</i> and the <i>iTol2</i> cassette was introduced for germline transgenes. B. Identification of transgenic zebrafish by PCR with <i>m3ck</i> primers. HH-F/R primers derived from <i>tiggy winkle hedgehog</i> exon 3 were used as control primers. −, non-transgenic fish; +, transgenic fish; wt, embryo of wild-type fish; G, genomic DNA of wild-type zebrafish; P, the transgenic plasmid <i>iTol2</i>-<i>smyd1</i>-<i>m3ck</i> as positive control; B, blank control.