The expression of cyst wall protein transcripts and the amount of cysts are different among <i>dhhc</i> transgenic encysting parasites.

<p>(A) qRT-PCR analysis of <i>cwp1, cwp2</i>, and <i>cwp3</i> transcripts expression in <i>dhhc</i> transgenic parasites after 48 h of encystation (white bars), relative to the expression in wild-type encysting cells (control) (black bars). The data are the means and SEM of three separate experiments, and each experiment was carried out in triplicate. (B) Percentage of water-resistant cysts in <i>dhhc</i> transgenic parasites determined by flow cytometry after 48 h of encystation. The results are presented as the percentage (mean ± SEM) of cysts in three independent experiments. The asterisks indicate that there was significant difference compared with the control (Student's t test: * p<0.05; **p<0.01; ***p<0.001).