Effects of the nor-NOHA on diet-induced obesity and liver weight.

<p>(A) body weight, (B) body weight gain, (C) epididymal, perirenal, retroperitoneal, and mesenteric fat weight, (D) liver weight, (E) epididymal fat morphology, and (F) liver morphology in mice fed with normal or high-fat diets (ND and HFD, respectively). C57BL/6 mice were fed an ND or HFD for 12 weeks. In HFD-fed mice, after 7 weeks of obesity induction, the animals were placed on either only HFD or HFD orally gavaged with 40 mg kg⁻¹ nor-NOHA (arginase inhibitor) dissolved in 0.9% NaCl solution for 5 weeks. Body weight was measured weekly. Food intake per mouse was recorded every other day. Representative liver and epididymal fat paraffin sections were stained with hematoxylin and eosin from 3 groups: (i) ND, (ii) HFD, and (iii) HFD with nor-NOHA. Images were captured at 400× magnification. Scale bar, 200 µm. The results are expressed as means ± SEs of mice, and groups were compared by analysis of variance (ANOVA) with Duncan’s multiple range test. The same letter indicates no significant difference between two groups (p<0.05).