rBCG prime and MVA-Gag boost immune responses.

<p>Mice were primed with BCG[pWB206] or BCG[pHS207] (intraperitoneal vaccination; doses of 10⁷ CFU, 10⁵ CFU, 10³ CFU) or left unprimed, then boosted on day 56 with MVA-Gag (intramuscular vaccination, 10⁷ pfu). Splenocytes pooled from a group of 5 mice were used on day 68 in <b>A.</b> an IFN-γ ELISPOT assay with the GFPCD8 peptide, GagCD8 peptide or GagCD4 peptide. Bars represent the average number of SFU/10⁶ splenocytes ± the standard deviation of triplicate reactions after subtraction of average background responses of not more than 20±10 SFU per 10⁶ splenocytes. <b>B.</b> Splenocytes were cultured (48 h) with the GFPCD8 peptide, GagCD8 peptide or GagCD4 peptide and cytokine levels in the culture supernatant were quantified using a Th1/Th2 cytokine bead array assay and flow cytometry. Only IFN-γ was detected in the culture fluid. Levels are expressed as pg/10⁶ splenocytes and are from a representative experiment.