Effects of IGF-1 and PD98059 Induction on Proliferation, Invasion, and Cyr61 Expression in MCF-7 WT and MCF-7 CYA.

<p>(<b>A</b>) The mRNA expression of Cyr61 was assessed by RT-Q-PCR. All mRNA levels were adjusted for the housekeeping gene, 18S. (<b>B</b>) Proliferation assay summary data after 48 hour IGF-1 (100 ng/mL) and PD98059 (30 µM) treatment in MCF-7 WT and MCF-7 CYA. Each condition was plated into 6 identical wells in a 96 well plate. All treatments were standardized relative to the untreated condition for each cell line. (<b>C</b>) Results from Invasion assay after 40 hour IGF-1 (100 ng/mL) and PD98059 (30 µM) treatment in MCF-7 WT and MCF-7 CYA. P-values were assessed relative to the untreated condition in each assay, with P<0.05 considered statistically significant.