Role of PTPD1 in the spatial regulation of EGFR phosphorylated species (a) Quantitative PCR (left bar graph) and western blot (right panels) to evaluate the modulation of PTPD1 caused by the siRNA N21 transfection.

<p>Quantification of the extent of protein down modulation is shown. (b) FLIM to assess the phosphorylation of EGFR in MCF7-EG cells non-stimulated or stimulated 5 min with EGF and transfected with N21 or non-targeting (NT) siRNA pools. The GFP and phospho-tyrosine fluorescence, as well as maps of the GFP lifetime of a confocal section are shown in each condition. Lower panels show zooms of the areas labelled with white squares placed in lifetime maps. Arrowheads point discrete areas of EGFR phosphorylation. Calibration bar of the GFP fluorescence lifetime in nanoseconds (ns) is shown. EGFR-GFP phosphorylation is indicated by the decrease in the fluorescence lifetime of the GFP.