Calcium, IP1 and luciferase assay responses in WT MAS stable cell line.

<p>Dose dependent changes in (A) calcium flux and (B) IP1 levels in cells upon stimulation with AR-<i>agonist</i>, AR-<i>inverse agonist</i> (AR-<i>inverse</i>) and NPFF in induced WT stable cell line. (C) Complete inhibition of AR-<i>agonist</i> and NPFF dose-response curves upon pre-treatment with 25 µM of AR-<i>inverse agonist</i> (AR-<i>inverse</i>) in calcium assays. (D) Dose dependent changes in luciferase expression upon stimulation with AR-<i>agonist</i>, AR-<i>inverse agonist</i> (AR-<i>inverse</i>) and NPFF in induced WT stable cell line. Representative curves from a single experiment wherein measurements are made in triplicate are shown as mean±SEM. The number of independent experiments is: N> = 3 in panels A, B and D; N = 2 in panel C.