Inhibition of autophagy or over-expression of β-catenin attenuates the effects of resveratrol on BCSCs.

<p>(A) Western blot analysis of β-catenin and cyclin D1 in BCSCs treated with different dose of resveratrol for 24 h. (B) Immunohistochemistry detection of β-catenin and cyclin D1 in xenografted breast tumors. (C) The effects of resveratrol treatment for 24 h on ALDH-positive populations in SUM159 cells and SUM159 cells co-treated with 10 µM CQ (CQ-SUM). (D) The effects of resveratrol treatment for 24 h on autophagy in BCSCs, BCSCs co-treated with 10 µM CQ (CQ-CSCs) and BCSCs transfected with pcDNA3-S33Y β-catenin (TC_β-catenin). (E) The effects of resveratrol treatment for 24 h on cells viability in BCSCs, CQ-CSCs and TC_β-catenin cells. (F) Western blotting analysis of β-catenin and cyclin D1 in TC_β-catenin cells treated with different dose of resveratrol for 24 h. Data are presented as mean ± SD (<i>n</i> = 3). <b>^*</b><i>P</i><0.05, <b>^**</b><i>P</i><0.01 compared with the control (0 µM); <b>^#</b><i>P</i><0.05, <b>^##</b><i>P</i><0.01 compared with corresponding group in CSCs.