Temporal quantitative gene expression pattern during cardiomyogenesis.

<p>Graphs show real-time RT-PCR data showing various hallmark markers for cardiomyocyte differentiation (day 0, 1, 2, 4, 6, 8, 14 and 18). The mean Ct values of duplicate measurements were calculated and subsequently normalized against housekeeping gene (GAPDH) for the same sample. After normalization, the means of triplicate samples from three independent experiments were plotted relative to the day 0 for undifferentiated markers and with day 1 for differentiated markers. Data represented are mean ± SEM (n = 3).