Schematic of EzrA deletion mutants employed in this study.

<p>The predicted coiled-coil structure of EzrA is drawn to scale. Numbers refer to amino acid positions. Coiled-coil regions are shaded blue. The QNR motif is shaded yellow and circled in the full-length protein. Lines indicate deleted regions. The QNR domain construct missing all four coiled-coils is just under the full-length protein. All constructs used for <i>in vivo</i> analysis were fused in-frame to a C-terminal GFP moiety (not shown) to simplify analysis. All constructs used for <i>in vitro</i> analysis were fused in-frame to a N-terminal Thio tag to enhance solubility and a N-terminal 6x-His tag for purification purposes (not shown).