Bcl-2 and cell proliferation assay of embryonic cells under normoxia and hypoxia.

<p>(A). Bcl-2 assay: Bcl-2 reached its highest (p<0.05) level in cell lysates during 24 hpf. Bcl-2 expression was not detected in normoxic embryos during embryonic development. (B).Embryonic cell proliferation: Normoxic embryos showed continuous cell proliferation till 24 hpf, whereas hypoxic embryos showed no significant cell proliferation up to 7 hpf (gastrula phase), followed by abrupt increase in proliferation after 7 hpf up to 24 hpf. Different superscripts indicate significant difference (<i>p</i><0.05) amongst normoxic and hypoxic groups at different times. Values are expressed as mean ± SEM (n = 10).