Concentration dependence of cytosolic extract-mediated chromatin assembly.

<p>A) Varying amounts of yeast cytosolic extract were incubated with core histones and a relaxed, circular plasmid for 1 hour in the presence or absence of rHif1p (as indicated). Following removal of protein, DNA was isolated and resolved by agarose gel electrophoresis and visualized by staining with Sybr Gold. Migration of relaxed (R) and supercoiled (S) forms of the plasmid are indicated. B) Yeast cytoplasmic extract was assayed for chromatin assembly as above with either rHif1p or BSA, as indicated (left panel). The amounts of rHif1p and BSA used in the chromatin assembly assay were resolved by SDS-PAGE and stained with coomassie blue (right panel). For both the left and right panels, all of the lanes were run on the same gel but an irrelevant lane was removed from the figure.