Morphine increases neuronal differentiation and inhibits self-renewal of NPCs.

<p>NPCs exposed to increasing levels of morphine demonstrated a decrease in BrdU- positive- nestin-positive cell expression while TUJ1- positive- BrdU- positive co-staining was enhanced with increased morphine concentrations (*p<0.05, **p<0.01, ***p<0.001 vs. untreated; ⁺p<0.05, ⁺⁺⁺p<0.001 vs. 13 µM) (<b>3A, 3B</b>).