Morphologic analyses of NB4MTOE cells and their control cells.

<p>(A) NB4MTOE (NB4MT22, 23, 25) cells and their control (NB4pcDNA4, 6, 7) cells were collected and subjected to Wright–Giemsa staining. The magnification of the images shown in the panels is ×1000. Arrows indicate cytoplasmic granules. (B) Differential counts of NB4MTOE cells and their control cells in the presence or absence of ATRA. Cells were treated with 1 µM ATRA or solvent (control) for 5 days. After collection, the cells were subjected to Wright–Giemsa staining, and classified. This experiment was performed under blind labeling of each sample, with two licensed examiners. The two examiners counted more than 200 cells for each sample and obtained similar results. The percentages of the cells are the mean values of the data from the two examiners. (C) Aliquots of cells with or without ATRA treatment shown in panel B were examined for morphological changes by Wright–Giemsa staining. The magnification of the images shown in the panels is ×400.