The effects of CO on STAT3 glutathionylation.

<p><b>A.</b> and <b>B.</b> ECs were treated with 100 µM BioGEE for one hour and loaded with TCDC or MC for the indicated times to induce protein glutathionylation. <b>C.</b> and <b>D.</b> ECs were exposed to TCDC or MC for the indicated periods. S-glutathionylated STAT3 was immunoprecipitated and detected using an antibody against protein-SSG. The quantification of the band intensities was normalized to the control values. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100677#s3" target="_blank">Results</a> are presented as the mean ± SEM (n = 3). *<i>P</i><0.05 compared with untreated ECs.