MMP expression in CCl₄ induced liver injury with α-CD147 antibody intervention.

<p>Cirrhosis was induced in a mouse model of liver injury (<i>Balb/c</i>) with CCl₄. An antibody targeting CD147 (RL73.2) was given twice weekly for 4 weeks, mice given an equal amount of IgG were used as control. There were no appreciable changes in MMP expression with either α-CD147 antibody or IgG control treatment in the absence of CCl₄ (not shown). Tissue hydroxyproline was significantly increased with α-CD147 antibody treatment (Panel A). Compared with control MMP expression is significantly increased in the CCl₄ injury with IgG group and reversed by intervention with α-CD147 antibody (Panels B–E). TIMP-1 expression was upregulated in injury but unaffected by intervention with α-CD147 antibody (Panel F) (n = 5−7 per group data expressed as mean and SEM. *p<0.05, **p<0.01 and ***p<0.001).