Proliferation and transient activation of total B cells.

<p>Spleens and CLN from YFP-MuHV-4 infected SW_HEL mice were harvested at 0, 7, 14 and 21 dpi and cells were stained with CD69 PE, CD19 APC-Cy7 and HEL-A647. (A) Live cells were enumerated, and the number of HEL⁺ and HEL⁻ B cells was established based on their frequency obtained from the FACS data. (B) Representative FACS histograms showing CD69 expression on HEL⁺ (grey curves) and HEL⁻ (white curves) B cells isolated from CLN and spleen at the different time points are shown. CD69 was monitored by measuring the Geo MFI and compiled values are shown in the graphic below. (C) On the same samples, the frequency of CD69^high cells was evaluated. Representative FACS plots are shown and compiled percentages are presented in the graphic below. These data were obtained from two independent experiments, with a total 5 to 6 mice per time point. In the graphics, mean values are reported and error bars represent the standard deviation.