HEL⁺ and HEL⁻ B cells are both infected by MuHV-4 in vitro.

<p>In vitro infection by co-culture assay was performed as described in the material and methods. Briefly, freshly isolated SW_HEL splenocytes were exposed to free viruses or co-culture with BHK-21 or RAW-264 cells previously infected with EF1α-eGFP⁺ or EF1α-eGFP⁺-gp150⁻ MuHV-4. GFP expression in HEL⁺ and HEL⁻ B cells was monitored by FACS after 48 h of co-culture. Representative FACS plots are shown on top, compiled data representing average percentage and standard deviation are shown below. Splenocytes were identified based on FSC SSC parameters, excluding BHK-21 and RAW-264. Cells were then gated on CD19⁺ CD11b⁻ and GFP expression was monitored in HEL⁺ and HEL⁻ B cell. Data were obtained from two independent experiments with two splenocytes suspensions in each.