<p>(<b>A</b>) Yeast strains from Fig. 6A were treated with 0.5 µg/ml SM for 120 min and aliquots of WCE containing 1 mg total protein were immunoprecipitated with α-Gcn4 antibodies. Immunocomplexes were subjected to Western analysis with α-Gcn4 and α-Smt3 (sumo) antibodies. Relevant molecular weight markers (SeeBlue Pre-Stained Standard) are indicated. * indicates a nonspecific band observed even in the <i>gcn4Δ</i> strain. Gcn4 Western signals do not necessarily reflect the relative Gcn4 levels in the starting WCEs presumably owing to variability in the efficiency of immunoprecipitating Gcn4. (<b>B</b>) Western signals for sumoylated Gcn4 from the lower panel of (A) were normalized to the Gcn4 signals from the upper panel of (A) and the Me...
<p>(A) Asd4 physically interacts with the <i>GLN1</i> promoter <i>in vivo</i> following growth on GM...
in S. cerevisiae. Cultures were grown to mid-log phase, and normalized by cell density. 3 μL drops o...
<p><b>(A)</b> Transformants of <i>gcn2Δ</i> strain H1149 containing derivatives of low-copy plasmid ...
<p>(<b>A</b>) WT (BY4741) and <i>hom6Δ</i> (YR001) strains transformed with vector (pRS313) and <i>h...
<p>(<b>A</b>) WCEs were prepared from transformants of <i>gcn2</i>Δ strain HQY132 bearing high-copy-...
<p>(<b>A</b>) WT (BY4741), <i>gcn4Δ</i> (F731), <i>hom6Δ</i> (YR001), <i>pho85Δ</i> (F947), <i>hom6Δ...
<p>(<b>A</b>) Comparison of RNA-seq and Ribo-seq RPKM changes in <i>uba4Δ</i>. <i>GCN4</i> targets a...
<p>(<b>A</b>) Yeast was grown to saturation in selective media. 5-fold serial dilutions were spotted...
<p><b>(A)</b> HEK293 cells were co-transfected, immunoprecipitated (IP), and analyzed by western blo...
<p>(<b>A</b>) Ribo-seq and RNA-seq RPKMs for the <i>GCN4</i> open reading frame. Standard deviations...
(A) The Cek1 MAPK is hyper-activated by transforming wild-type cells with a hyperactive allele of ST...
<p>A) Growth of <i>trm6-504/MTR4</i> transformant bearing high-copy plasmid-born <i>(</i>HC) wild-ty...
A: The graph shows the result of the analysis obtained from the statistical comparison (T-test) for ...
<p>(<b>A</b>) Schematic diagram of the threonine biosynthesis pathway in <i>Saccharomyces cerevisiae...
<div><p>We screened the genome-wide set of S. cerevisiae knockout mutants by staining with anti-β-gl...
<p>(A) Asd4 physically interacts with the <i>GLN1</i> promoter <i>in vivo</i> following growth on GM...
in S. cerevisiae. Cultures were grown to mid-log phase, and normalized by cell density. 3 μL drops o...
<p><b>(A)</b> Transformants of <i>gcn2Δ</i> strain H1149 containing derivatives of low-copy plasmid ...
<p>(<b>A</b>) WT (BY4741) and <i>hom6Δ</i> (YR001) strains transformed with vector (pRS313) and <i>h...
<p>(<b>A</b>) WCEs were prepared from transformants of <i>gcn2</i>Δ strain HQY132 bearing high-copy-...
<p>(<b>A</b>) WT (BY4741), <i>gcn4Δ</i> (F731), <i>hom6Δ</i> (YR001), <i>pho85Δ</i> (F947), <i>hom6Δ...
<p>(<b>A</b>) Comparison of RNA-seq and Ribo-seq RPKM changes in <i>uba4Δ</i>. <i>GCN4</i> targets a...
<p>(<b>A</b>) Yeast was grown to saturation in selective media. 5-fold serial dilutions were spotted...
<p><b>(A)</b> HEK293 cells were co-transfected, immunoprecipitated (IP), and analyzed by western blo...
<p>(<b>A</b>) Ribo-seq and RNA-seq RPKMs for the <i>GCN4</i> open reading frame. Standard deviations...
(A) The Cek1 MAPK is hyper-activated by transforming wild-type cells with a hyperactive allele of ST...
<p>A) Growth of <i>trm6-504/MTR4</i> transformant bearing high-copy plasmid-born <i>(</i>HC) wild-ty...
A: The graph shows the result of the analysis obtained from the statistical comparison (T-test) for ...
<p>(<b>A</b>) Schematic diagram of the threonine biosynthesis pathway in <i>Saccharomyces cerevisiae...
<div><p>We screened the genome-wide set of S. cerevisiae knockout mutants by staining with anti-β-gl...
<p>(A) Asd4 physically interacts with the <i>GLN1</i> promoter <i>in vivo</i> following growth on GM...
in S. cerevisiae. Cultures were grown to mid-log phase, and normalized by cell density. 3 μL drops o...
<p><b>(A)</b> Transformants of <i>gcn2Δ</i> strain H1149 containing derivatives of low-copy plasmid ...