BIX01294 enhances imatinib- and IFNα2a-induced apoptosis in K562 cells.

<p>(<b>A</b>) K562 cells were cultured with various concentrations of BIX01294 and imatinib as indicated. After four days, cell proliferation was measured with a MTT assay. Results represent the mean ± SD in quadruplicate experiments. (<b>B</b>) K562 cells were treated with or without IFNα2a (15 k IU/ml), or imatinib (150 nM) in the presence or absence of BIX01294 (2 µM) for 2 days. Cells were washed with PBS and fixed with 70% ethanol. Fixed cells were then stained with PI and analyzed with FACS. (<b>C</b>) K562 cells were stimulated with or without IFNα2a (10 k IU/ml), or imatinib (75 nM) in the presence or absence of BIX01294 (2 µM) for 2 days. Whole cell extracts were prepared and subjected to immunoblotting using the indicated antibodies.