Mapping the <i>C. crescentus</i> coding DNA sequence architecture.
- Jared M. Schrader (607038)
- Bo Zhou (15092)
- Gene-Wei Li (607039)
- Keren Lasker (334875)
- W. Seth Childers (607040)
- Brandon Williams (607041)
- Tao Long (266065)
- Sean Crosson (115248)
- Harley H. McAdams (607042)
- Jonathan S. Weissman (509877)
- Lucy Shapiro (225017)
Publication date
July 2014
DOIAbstract
<p><b>A.</b> Mapping of the correct start codon for ftsA. Shown below are phase contrast images of cells containing a high copy plasmid with either the newly identified start codon (pBX<i>ftsA</i>) or the old start codon (pBX-<i>ftsA</i>Δ<sub>N1-18</sub>) grown in M2G before and after induction with xylose for 6 hours. Scale bar is 6.05 µm. <b>B.</b> Putative small leader CDS identified on the <i>trpS</i> mRNA. <b>C.</b> Ribosome profiling identification of an intergenic small CDS. <b>D.</b> Alternative translation initiation site identified in the <i>CCNA_02448</i> mRNA allows translation of two in-frame protein isoforms. Internal start codon was verified by LC-MS.</p
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