Specific depletion of human pDC in lymphoid organs in vivo with a human pDC-reactive monoclonal antibody.

<p>Humanized Mice were treated with either 15B or isotype control (iso) antibody for 3 times on days -5, -3, -1 prior to termination. Percentages of pDC (Lin⁻CD4⁺CD123⁺) in total human leukocytes (CD45⁺) are analyzed. (<b>A</b>) Representative FACS plots and summarized data show relative pDC frequencies before and after antibody treatment in the peripheral blood (n = 7). (<b>B</b>) Representative FACS plots and summarized data show pDC depletion by 15B in mesenteric lymph nodes (mLN) and spleen (SP, isotype n = 4; 15B n = 5). All bars in dot graphs indicate median value. Error bars indicate standard deviations (SD). * and ** indicate p<0.05 and p<0.01, respectively. (<b>C</b>) Summarized percentages of CD3⁻CD19⁺ B cells in different lymphoid tissues. (<b>D</b>) Summarized percentages of CD3⁺CD19⁻ T cells in different lymphoid tissues. (<b>E</b>) Summarized percentages of CD3⁻CD11c⁺ mDC cells in different lymphoid tissues. All bars in dot graphs indicate median value.