Determination of intracellular oxidative stress indices within macrophages.

<p>(<b>A–C</b>) Level of oxidative stress indices in macrophages treated with combination of β-hematin (60 µg/ml)/methemoglobin (7.75 µM) over the course of time. Macrophages were either untreated or treated with the combination of β-hematin (60 µg/ml)/MetHb (7.75 µM) for different time points (0–6 hr) and (<b>A</b>) lipid peroxidation (<b>B</b>) protein carbonyl and (<b>C</b>) reduced glutathione is measured and expressed as mM/mg of cell lysate. Data is the mean ± SD of three independent experiments (n = 3) with triplicate measurement. Cellular viability is measured to correlate the change in oxidative stress with the viability of treated cells. The correlation factor (r²) for change in viability Vs lipid peroxidation is 0.97, change in viability Vs protein carbonyl is 0.86 and change in viability Vs GSH level is 0.97.