Identification of chitin-binding and –degrading proteins in the supernatants of cultured <i>P. larvae</i>.

<p>(A) Chitin-binding proteins isolated via chitin-beads from culture supernatants of ATCC9545 (<i>P. larvae</i> ERIC I) and DSM25430 (<i>P. larvae</i> ERIC II) were subjected to SDS-PAGE analysis. <i>Pl</i>CBP49 is marked by an arrowhead. (B) Proteins isolated via chitin-beads from culture supernatants of ATCC9545 (<i>P. larvae</i> ERIC I) and DSM25430 (<i>P. larvae</i> ERIC II) were subjected to zymography using ethylene glycol chitin- (EGC-) impregnated gels to assess their chitinolytic activity. <i>Pl</i>CBP49 is marked by an arrow head.