Identification of the mAb TRA 54 and pAb anti-albumin-reactive protein in mouse caput epididymis by immunoprecipitation followed by immunoblotting.

<p>Detection of TRA 54-reactive protein in caput epididymis homogenates. Aliquots (50 µg) of the homogenate was loaded (lane 1). The immunoprecipitate (IP) obtained with IgGs was probed (IB) with mAb TRA 54 (lane 2). The immunoprecipitate obtained with pAb rabbit anti-albumin (AA) IgG (Abcam; ab34807) was probed with mAb TRA 54 (lane 3) or with goat pAb against the N-terminal of albumin (Santa Cruz Biotechnology; sc-46291) (lane 4). The immunoprecipitate obtained with mAb TRA 54 was probed with rabbit anti-albumin IgG (Abcam; ab34807) (lane 5) and mAb TRA 54 (lane 6). For immunoprecipitation, Dynabeads-protein G (Veritas, Tokyo, Japan) were used according to the manufacturer’s recommendation. Arrows indicate the signals. Molecular mass markers are indicated on the left.