MyD88 is crucial for the resolution of non-severe sepsis.

<p>a–g) Non-severe (NS) and severe (S) sepsis were induced by CLP in WT and <i>MyD88</i>-deficient mice and 6 or 24 h after sepsis induction, the following were assessed: a) neutrophil recruitment to the peritoneal cavity; b) CXCL1 and c) CXCL2 levels in the peritoneal lavage determined by ELISA; d) bacterial count in the peritoneal lavage and e) blood; f) IL-6 levels in the plasma, as measured by ELISA and g) lung MPO activity. h) Peritoneal macrophages were stimulated with LPS (1 µg/mL) or LTA (10 µg/mL) for 24 h and CXCL2 and i) TNF-α were measured in the supernatant by ELISA. These data are expressed as the mean ± SEM in <i>a, b</i>, <i>c, f</i>, <i>g, h</i> and <i>i</i> and median in <i>d</i> and <i>e</i>. The data were analyzed by multifactorial ANOVA followed by unpaired <i>t test</i>. The graphs are representative of one to four independent experiments. <i>n = </i>4 to 10<i>; ND = </i>not detected.*<i>P</i><0.05.