CXCR7 is induced by TNF-α in a time and dose-dependent manner.

<p>HBMECs were treated with 10/ml of TNF-α and collected at 0, 1, 3, 6, or 16 hours or treated with increasing concentrations (1, 10, 100 ng/ml) of TNF-α for 24 hours. <i>A, B,</i> and <i>D,</i> Total RNA from TNF-α-treated HBMECs was analyzed by qPCR as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103938#s2" target="_blank">Materials and Methods</a>”. Data are shown as means ± S.D from three independent experiments. *, <i>p</i><0.05; **, <i>p</i><0.01. <i>C</i> and <i>E,</i> Total protein from TNF-α-treated HBMECs was analyzed by Western blot as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103938#s2" target="_blank">Materials and Methods</a>”. Three independent experiments were performed and representative data are shown.