CD spectra and SLS analysis of MBP-Orf mutant proteins.
- Fiona A. Curtis (482276)
- Ali D. Malay (482275)
- Alexander J. Trotter (607358)
- Lindsay A. Wilson (607359)
- Michael M. H. Barradell-Black (607360)
- Laura Y. Bowers (607361)
- Patricia Reed (607362)
- Christopher R. T. Hillyar (607363)
- Robert P. Yeo (607364)
- John M. Sanderson (607365)
- Jonathan G. Heddle (482278)
- Gary J. Sharples (482277)
Publication date
August 2014
DOIAbstract
<p>(<b>A</b>) SLS analysis of selected MBP-Orf mutant proteins. Proteins at 0.2 mg/ml in 100 mM Tris-HCl pH7 at 25°C were analyzed in a Zetasizer µV. (<b>B</b>) CD analysis of MBP-Orf mutant proteins. CD spectra (180-260 nm) were obtained for MBP-Orf proteins in ultrapure water at 20°C and analysis performed using CDSSTR, Contin/LL and Selcon3 programs from the CDPro suite <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102454#pone.0102454-Sreerama1" target="_blank">[54]</a>.</p
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