Ginsenoside Rb3 suppresses OGD-Rep-induced NF-κB activation in H9c2 cells.

<p>H9c2 cells were treated with ginsenoside Rb3 at different concentration (5 µM or 2 µM) followed by OGD-Rep. The cells were harvested and subjected to ELISA and Western blot assays. (A) The NF-κB activity was analyzed using a NF-κB p65 ELISA Kit, and the graph represented the ratio of the nuclear to the cytoplasmic p65 concentration. (B) The NF-κB p65 protein levels and NF-κB p65 phosphorylation were analyzed by Western blot assay. Beta-actin was used as an internal control. (C) The IκB-α protein levels and IκB-α phosphorylation were determined by Western blot assay, and beta-actin was used as an internal control. The graph represented the ratio of phosphorylated IκB-α to beta-actin. Ginsenoside Rb3 blocked OGD-Rep-induced increase in IκB-α phosphorylation levels. All data were shown as the mean ± SD. ^#P<0.05 vs. group without OGD-Rep. *P<0.05 vs. group treated with OGD-Rep alone. n = 4–6.