Construction of a quality control system.

<p>(A) A schematic diagram for the subcloning of the 3′-UTR of <i>Lin28</i> and <i>ERBB2</i> into the pGL3 vector. (B) HEK293T cells were co-transfected with Let-7b or miR-125a and the 3′-UTR of <i>Lin28</i> or <i>ERBB2</i> for the dual-luciferase assay. PRL-TK plasmid expressing the <i>Renilla</i> luciferase was used as a transfection control. The luciferase activity was detected 48 h after transfection, and the results were analyzed using the Student’s t-test. *<i>P</i><0.05, **<i>P</i><0.01.