PRKCD and PRKCE are both expressed in mouse embryonic endothelium at E9.5.

<p>A, whole mount LacZ staining suggests weak expression of PRKCD in few endothelial cells. B, PRKCE appears broadly expressed in endothelium in whole mount LacZ stained embryos. C and D, LacZ and eosin stained sections confirmed the endothelial expression pattern observed for PRKCD and PRKCE in whole embryos, respectively. E, western blotting shows that wild type mouse embryonic endothelial cells (MEECs) express PRKCD. <i>Prkcd</i>−/− MEECs were used as a negative control. F, CD31 positive signal in virtually all MEECs confirms the purity of WT and <i>Prkcd</i>−/− cell populations. Mouse embryonic fibroblasts (MEFs) were used as a negative control. Expression of PRKCE in MEECs was previously demonstrated in a similar manner <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103686#pone.0103686-Carracedo1" target="_blank">[20]</a>. Scale bars = 500 µm (A and B), 25 µm (C and D) and 200 µm (F).