Flow cytometric analysis of profilin 1 clones and its mutants reveal overexpression of profilin.
- Wasia Rizwani (369727)
- Aneesa Fasim (607546)
- Deepshikha Sharma (607547)
- Divya J. Reddy (607548)
- Nabil A. M. Bin Omar (607549)
- Surya S. Singh (607550)
Publication date
August 2014
DOIAbstract
<p>(A) Western blot showing overexpressed profilin 1 and its mutants (41 KDa), endogenous profilin 1 (14 kDa) and actin (42 KDa). (B) Phase contrast image showing well spread out monolayer of transfected MCF7 cells (upper panel) and rounded nuclei alone at 2 min and 10 min (middle and bottom panels). Magnification-20×. Flow cytometric analysis showing green fluorescent positive nuclei from (C) EV, (D) PFN-WT, (E) PFN-S137A, (F) PFN-R74E and (G) PFN-H133S clonal populations.</p
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