Administration of late-passage hMSCs reduces the protective effects against DSS-induced colitis in mice.

<p>(A–F) 3% DSS water was administered to mice for seven days to induce colitis. Early- or late-passage hMSCs were injected intraperitoneally one day after the administration of DSS. The percentage of body weight loss (A), the Mantel Cox analysis of survival rate (B) and the disease activity index for colitis severity (C) were monitored as clinical progression. Mice were sacrificed ten days after the induction of colitis with DSS, and the length (D), weight-to-length ratios of the colons (E) and colon sections were stained with H&E and histopathologic evaluation was investigated by determining lymphocyte infiltration and intestinal damage (F, G). (*<i>P</i><0.05, **<i>P</i><0.01).