Screening and typing of 12 kinds of β-thalassemia mutations in Chinese by HRM analysis.

<p>The arrowheads indicated different genotypes. <b>A, C, E, G, I</b> and <b>K</b> were the Temp-shift melting curves; <b>B, D, F, H, J</b> and <b>L</b> were the Temp-shift difference plots. Ht: heterozygote; Ho: homozygote; c.-78&79 A>G Ht: c.-78 A>G heterozygote or c.-79A>G heterozygote. <b>A</b> and <b>B</b>: HB01 primer set for c.-78&79 A>G Ht and c.-78 A>G Ho; <b>C</b> and <b>D</b>: HB02 primer set for c.45_46insG Ht, c.52A>T Ht and c.52A>T Ho; <b>E</b> and <b>F</b>: HB03 primer set for c.79G>A Ht, c.84_85insC Ht, c.92+1G>T Ht and c.316–197C>T Ht; <b>G</b> and <b>H</b>: HB04 primer set for c.126_129delCTTT Ht; c.130G>T Ht and c.126_129delCTTT Ho; <b>I</b> and <b>J</b>: HB05 primer set for c.216_217insA Ht; <b>K</b> and <b>L</b>: HB06 primer set for c.316–197C>T Ht and c.316–197C>T Ho.